Experimental Study Acute toxic effects of benzamide derivative on renal histomorphology of BALB-C mice

Abstract

ABSTRACT

Introduction: For developing a new drug, it must be tested successively on animals and humans for its efficacy and side effects. This includes a series of studies to establish the safety of drug. In these studies, the effects are observed in certain organs like brain, heart, lungs, kidneys. GIT, liver, and reproductive organs. The present study was performed on Balb-C mice to see the toxic effects of benzamide derivative on the histomorphology of kidneys.

Materials & Methods: This experimental study was carried out at Institute of Basic Medical Sciences (IBMS), Khyber Medical University (KMU) Peshawar. Forty male BALB/c mice were used in this study. The benzamide derivative was administered orally in incremental doses.

Results: By increasing the dose of benzamide derivative up to 100mg/kg, toxicity induced histopathological changes were observed in the kidneys of Balb-C mice in the form of lymphocyte infiltration, increase in urinary space, glomerulosclerosis, peri glomerulosclerosis, tubular swelling, tubular necrosis, and vascular congestion.

Conclusion: Before a drug is declared safe for human consumption, safety studies are carried out in animals. This compound (benzamide derivative) causes toxicity in kidneys with increasing doses.

Keywords: drug testing, renal toxicity, animal studies for drugs, kidney histomorphological changes