Serum and vitreous levels of Interleukin-6, Leptin and Vascular Endothelial Growth Factor in patients with proliferative and non-proliferative diabetic retinopathy
Keywords:Interleukin-6, Leptin, Vascular Endothelial Growth Factor, Diabetic Retinopathy
Introduction: Retinopathy is one of the most dreaded consequences of chronic diabetes mellitus, progressively leading to blindness, and is believed to result from inflammatory and cytokine mediators.
Objective: To document the levels of Interleukin-6, Leptin and Vascular Endothelial Growth Factor in serum and vitreous fluid of patients with proliferative and non-proliferative diabetic retinopathy.
Materials & Methods: It was a comparative study conducted in main three hospitals of Peshawar and Al-Shifa Hospital Rawalpindi. Laboratory analyses were done in IRNUM, Peshawar. A total of 274 diabetics with and without retinopathy participated in the study. Serum and vitreous levels of the aforementioned parameters were measured by ELISA. Data analysis was done by using SPSS version 17; pâ‰¤0.05 was taken as significant.
Results: Serum and vitreous IL-6 levels were significantly greater in PDR and NPDR patients (p<0.001). Serum leptin concentrations were also significantly greater in PDR and NPDR patients as compared to DNR patients and normal subjects (p<0.001) but vitreous leptin concentrations did not differ significantly between NPDR and PDR patients (p=0.915). Serum VEGF values were elevated in DNR, NPDR and PDR patients as compared to the normal subjects (p<0.001). Vitreous level of VEGF was significantly raised in PDR than NPDR (p<0.001).
Conclusion: Patients with PDR had significantly high serum and vitreous levels of IL-6 and VEGF. Serum leptin levels were significantly high in PDR and NPDR but vitreous levels of leptin did not show significant difference in both PDR and NPDR.